44. Y. Zeng, M. Woolley, K. Chockalingam, B. Thomas, S. Arora, M. Hook, Z. Chen. Click display: a rapid and efficient in vitro protein display method for directed evolution. Nucleic Acid Research. DOI: 10.1093/nar/gkad643

43. Chonira V, Kwon YD, Gorman J, Case JB, Ku Z, Simeon R, Casner RG, Harris DR, Olia AS, Stephens T, Shapiro L, Bender MF, Boyd H, Teng IT, Tsybovsky Y, Krammer F, Zhang N, Diamond MS*, Kwong PD*, An Z*, Chen Z.* A potent and broad neutralization of SARS-CoV-2 variants of concern by DARPins. Nat Chem Biol. 2022. Epub 20221121. doi: 10.1038/s41589-022-01193-2. PubMed PMID: 36411391. (* Co-corresponding authors)

42. Zeng Y, Jiang M, Robinson S, Peng Z, Chonira V, Simeon R, Tzipori S, Zhang J,* Chen Z.* A Multi-Specific DARPin Potently Neutralizes Shiga Toxin 2 via Simultaneous Modulation of Both Toxin Subunits. Bioengineering. 2022;9(10):511. PubMed PMID: doi:10.3390/bioengineering9100511. (* Co-corresponding authors)

41. Woolley M, Chen Z. A PCR-free rapid protocol for one-pot construction of highly diverse genetic libraries. PLOS ONE. 2022;17(10):e0276338. doi: 10.1371/journal.pone.0276338.

40. Chockalingam K*, Kumar A, Song J, Chen Z*. Chicken-derived CD20 antibodies with potent B-cell depletion activity. Br J Haematol. 2022. Epub 20220830. doi: 10.1111/bjh.18438. PubMed PMID: 36039695. (* Co-corresponding authors)

39. Jiang M, Shin J, Simeon R, Chang JY, Meng R, Wang Y, Shinde O, Li P, Chen Z, Zhang J. Structural dynamics of receptor recognition and pH-induced dissociation of full-length Clostridioides difficile Toxin B. PLoS Biol. 2022;20(3):e3001589. Epub 20220324. doi: 10.1371/journal.pbio.3001589.

38. Simeon RA, Zeng Y, Chonira V, Aguirre AM, Lasagna M, Baloh M, Sorg JA, Tommos C, Chen Z. Protease-stable DARPins as promising oral therapeutics. Protein Eng Des Sel. 2021;34. doi: 10.1093/protein/gzab028.

37.Chockalingam, K., Peng, Z., Vuong, C.N., Berghman, L.R. & Chen, Z. Golden Gate assembly with a bi-directional promoter (GBid): A simple, scalable method for phage display Fab library creation. Scientific reports 10, 2888 (2020).

36. Heinzelman, P., Low, A., Simeon R.,Wright, G., Chen, Z., 2019. De Novo Isolation & Affinity Maturation of Yeast-Displayed Virion-Binding Human Fibronectin Domains by Flow Cytometric Screening Against Virions. Journal of Biological Engineering. 13: 76. (2019)

35. Peng, Z., Simeon R., Mitchell, S., Zhang, J., Feng, H., Chen, Z. Designed ankyrin repeat protein (DARPin) neutralizers of TcdB from Clostridium difficile Ribotype 027. mSphere. DOI: 10.1128/mSphere.00596-19 (2019).

34. Simeon R, Jiang M, Chamoun-Emanuelli AM, Yu H, Zhang Y, Meng R, Peng Z, Jakana J, Zhang J, Feng H, Chen Z.  Selection and characterization of ultrahigh potency designed ankyrin repeat protein inhibitors of C. difficile toxin B. PLoS Biol 17:e3000311. (2019)

33. Kasaraneni N, Chamoun-Emanuelli AM, Wright GA, Chen Z. A simple strategy for retargeting lentiviral vectors to desired cell types via a disulfide-bond-forming protein-peptide pair. Scientific reports.8(1): 10990. doi: 10.1038/s41598-018-29253-5 (2018).

32. Kasaraneni, N., Chamoun-Emanuelli, A. M., Wright, G. & Chen, Z. “Retargeting Lentiviruses via SpyCatcher-SpyTag Chemistry for Gene Delivery into Specific Cell Types”. MBio 8, doi:10.1128/mBio.01860-17 (2017).

31. Lee, G., Jaiswal, M., Gaharwar, A. K. & Chen, Z. “Versatile Click-Protein Hydrogels for Biomedical Applications”. Chemistry Select 2, 10310-10315 (2017).

30. Simeon, R. & Chen, Z.In vitro-engineered non-antibody protein therapeutics”. Protein & Cell, doi:10.1007/s13238-017-0386-6 (2017)

29. Ramirez, M.A. & Chen, Z. “Split-Intein Triggered Protein Hydrogels”. Methods Mol Biol 1495, 161-171 (2017).

28. Guan, D. & Chen, Z. “Affinity Purification of Proteins in Tag-Free Form: Split Intein-Mediated Ultrarapid Purification (SIRP)”. Methods Mol Biol 1495, 1-12 (2017).

27. Chen, Z., Rice, C. “Repurposing an old drug: A low-cost allergy medication provides new hope for hepatitis C patients”. Hepatology. 62, 1911-1913 (2015).

26. Chamoun-Emanuelli, A., Wright, G., Smith, R., Munch, R., Buchholz, CJ., Chen, Z., “In vitro incorporation of a cell-binding protein to a lentiviral vector using an engineered split intein enables targeted delivery of genetic cargo”. Biotechnology and Bioengineering. 112, 2611-2617 (2015).

25. Guan, D., Kurra, Y., Liu, W., Chen, Z., “A click chemistry approach to site-specific immobilization of a small laccase enables efficient direct electron transfer in a biocathode”. Chem Comm. 51, 2522-5 (2015).

24. Chamoun-Emanuelli, A., Pecheur, E-I., Chen, Z., “Benzhydrylpiperazine compounds inhibit cholesterol-dependent cellular entry of hepatitis C virus”. Antiviral Research. 109, 141-8 (2014).

23. Guan, D., Chen, Z., “Challenges and recent advances in affinity purification of tag-free proteins”. Biotechnology Letter. 36, 1391-406 (2014).

22. Chamoun-Emanuelli, A., Bobardt, M., Moncla, B., Mankowski, M. K., Ptak, R. G., Gallay, P., Chen, Z., “Evaluation of PD 404,182 as an anti-HIV and anti-HSV microbicide”. Antimicrobial Agents and Chemotherapy. 58, 687-97 (2014).

21. Simeon, R., Chen, Z., “A screen for genetic suppressor elements of hepatitis C virus identifies a supercharged protein inhibitor of viral replication”. Plos One. 8, e84022 (2013).

20. Simeon, R., Chamoun-Emanuelli, A., McMillan, T., Chen, Z., “Discovery and characterization of a new cell-penetrating protein”. ACS Chemical Biology. 8, 2678-87 (2013).

19. Guan, D., Ramirez, M., Shan, L., Jacobsen, D., Barrera, I., Lutkenhaus, J., Chen, Z., “Two-component protein hydrogels assembled using an engineered disulfide-forming protein-ligand pair”. Biomacromolecules, 14, 2909-2916 (2013).

18. Ramirez,M., Chen, Z., “Preparation of Intein-triggered artificial protein hydrogels”.  Journal of Visualized Experiments (JoVE) (83), e51202. (2014).

17. Ramirez, M., Guan, D., Ugaz, V., Chen, Z., “Intein-triggered artificial protein hydrogels that support the immobilization of bioactive proteins”. Journal of the American Chemical Society (JACS). 135, 5290-3 (2013).

16. Guan, D., Ramirez., Chen. Z. “Split intein mediated ultra-rapid protein purification (SIRP)”. Biotechnology and Bioengineering. 110, 2471-81(2013).

15. Chamoun-Emanuelli, A., Pecheur, E-I., Simeon, R., Huang, D., Cremer, PS., Chen, Z., “Phenothiazines inhibit HCV entry likely by increasing the fluidity of cholesterol-rich membranes”. Antimicrobial Agents and Chemotherapy (AAC). 57, 2571-81 (2013).

14. Zhao, Z., Ramachandran, P., Kim, T-S., Chen, Z., Jeya, M., Lee, J-K., “Characterization of an Acid-Tolerant β-1,4-Glucosidase from Fusarium oxysporum and its Potential as an Animal Feed Additive”. Applied Microbiology and Biotechnology (AMB) 97:10003-11 (2013)

13. Ramirez, M., Valdes, N., Guan, D., Chen, Z., “Engineering split intein DnaE from Nostoc punctiforme for rapid protein purification”. Protein Engineering Design Selection (PEDS) 26: 215-23 (2013)

12. Singh, R.K., Tiwari, M.K. Kim, I-W., Chen, Z.*, Lee, J-K.*,“Probing the Role of Sigma π Interaction and Energetics in the Catalytic Efficiency of Endo-1,4-β-Xylanase”. Applied and Environmental Microbiology, 78, 8817-8821 (2012) (* Co-corresponding authors)

11. Chamoun, A.M., Chockalingam, K., Bobardt, M., Simeon, R., Chang, J., Gallay, P., Chen, Z., “PD 404,182 is a Virucidal Small Molecule that Disrupts Hepatitis C Virus and Human Immunodeficiency Virus.” Antimicrobial Agents and Chemotherapy (AAC), 56, 672-681 (2012).

10. Chockalingam, K., Simeon, R., Rice, C. and Chen, Z., “A Cell Protection Screen Reveals Potent Inhibitors of Multiple Stages of the Hepatitis C Virus Life Cycle.” Proceedings of the National Academy of Sciences of the United States of America (PNAS), 107, 3764-3769 (2010).

9. Chen, Z.*, Simeon, R., Chockalingam, K. and Rice, C.*, “Creation and Characterization of a Cell-death Reporter Cell Line for Hepatitis C Virus Infection.” Antiviral Research, 86, 220-223 (2010). (* Co-corresponding authors).

(Prior to TAMU)
8. Chen, Z., Wen, F., Sun, N. and Zhao, H. “Directed Evolution of Homing Endonuclease I-SceI with Altered Sequence Specificity.” Protein Engineering, Design & Selection (PEDS).22, 249-256 (2009).
7. Chen, Z. and Zhao, H. “Protein Design.” In Encyclopedia of Chemical Processing, (S. Lee, Ed.) pp. 2467-2477, Marcel Dekker, Inc., New York, NY (2006).
6. Chen, Z. and Zhao, H. “A Highly Sensitive Selection Method for Directed Evolution of Homing Endonucleases.” Nucleic Acids Research (NAR), 33, e154 (2005).
5. Chen, Z. and Zhao, H. “Rapid Creation of a Novel Protein Function by in vitro Coevolution.” Journal of Molecular Biology (JMB), 348, 1273-1282 (2005).
4. Chockalingam, K., Chen, Z., Katzenellenbogen, J. A., and Zhao, H. “Directed Evolution of New Specific Receptor-Ligand Pairs for Use in the Creation of Gene Switches.” Proceedings of the National Academy of Sciences of the United States of America (PNAS), 102, 5691-5696 (2005).
3. Chen, Z., Katzenellenbogen, B. S., Katzenellenbogen, J. A. and Zhao, H. “Directed Evolution of Human Estrogen Receptor Variants with Significantly Enhanced Androgen Specificity and Affinity.” Journal of Biological Chemistry (JBC), 279, 33855-33864 (2004).
2. Chen, Z. and Zhao, H. “A Highly Efficient and Sensitive Screening Method for Trans-activation Activity of Estrogen Receptors.” Gene, 306, 127-134 (2003).
1. Zhao, H., Chockalingam, K., and Chen, Z. “Directed Evolution of Enzymes and Pathways for Industrial Biocatalysis.” Current Opinion in Biotechnology, 13, 104-110 (2002).