Current Projects

TRE diagram
Diagram of the two transgene "Tet-On" utlized in the lab's inducible in vivo models.

In vivo models of inducible protein expression

The laboratory makes use of in vivo models of inducible protein expression or deletion using the “Tet-On” system to manipulate cell biology and tissue physiology in the adult. The transgene system utilized promoters that are specific to a cell/tissue to regulate the localization of induction. In the above example, promoters that localize rtTA expression to adipose tissue, the kidney, or the lung are used to drive local overexpression of the lymphangiogenic murine protein VEGF-D. We can then study the effects of, in this case, local lymphatic hyperplasia on tissue fluid balance and organ function.

In vivo models of protein deletion

Using the classical cell-specific Cre system, we are targeting the basic cell biology of lymphatic enodthelial cells or the predominant cells in lymphatic-rich tissues in vivo to probe the impacts of cell signaling, transport, and/or metabolism on fluid and metabolite transport.

Research Funding

Project nameFunding sourceTypeCurrent statusPrincipal researcher
Inducible lymphatic hyperplasia to drive chylous accumulation mimicking lymphangiomatosisUniversity of Pennsylvania Orphan Disease Center Million Dollar Bike Ride Grant ProgramPilot GrantCompletedJoseph M. Rutkowski
Lymphatic circulation: regulator of adipose tissue metabolismAmerican Heart AssocationScientist Development GrantCompletedJoseph M. Rutkowski
Adiponectin transport: endothelial modulation of adipokine functionNIH/NIDDKPostdoctoral FellowshipCompletedJoseph M. Rutkowski